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JoVE Journal
Biochemistry

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Laboratory Scale Production and Purification of a Therapeutic Antibody
 

Laboratory Scale Production and Purification of a Therapeutic Antibody

Article DOI: 10.3791/55153
January 24th, 2017

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Summary January 24th, 2017

This protocol describes the production of a therapeutic antibody in a mammalian expression system. The methods described include preparation of vector DNA, stable transfection and serum-free adaptation of a human embryonic kidney 293 cell line, set up of large scale cultures and purification using affinity chromatography.

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