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January 24, 2017
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Mitochondriën kan de elektrochemische potentiaal over hun binnenste membraan (Δ Ψm) te gebruiken om calcium sekwestreren (Ca 2+), waardoor ze vorm te geven cytosolische Ca 2+ het signaleren binnen de cel. We beschrijven een werkwijze voor het gelijktijdig meten van mitochondriën Ca2 + opname en ΔΨ m in levende cellen met fluorescerende kleurstoffen en confocale microscopie.
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Cite this Article
McKenzie, M., Lim, S. C., Duchen, M. R. Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy. J. Vis. Exp. (119), e55166, doi:10.3791/55166 (2017).
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