Examination of Host Phenotypes in Gambusia affinis Following Antibiotic Treatment

The overall goal of this experimental system is to examine the negative side effects to a host organism after antibiotic treatment. So this method can help answer key questions in the microbiome field, such as enbac associated side effects on the host's health that is mediated by a disrupted microbiome. So the main advantages of this technique are that this system is straightforward, inexpensive in examining vertebrate hosts'physiological phenotypes, and allows for noninvasive disruption to the mucosal microbiome.

This technique's implications characterize how a disruption in the microbiome of an animal can lead to major side effects. To begin, streak a previously obtained and frozen infectious strain of Edwardsiella ictaluri onto E.Ictaluri selective and differential medium agar. Incubate the culture at 27 degrees Celsius for two days.