JoVE Journal
Developmental Biology
Developmental Biology
A subscription to JoVE is required to view this content.
Chapters
Summary
Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.