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Swab Sampling Method for the Detection of Human Norovirus on Surfaces
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Immunology and Infection
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JoVE Journal Immunology and Infection
Swab Sampling Method for the Detection of Human Norovirus on Surfaces

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

DOI:
10.3791/55205-v

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10:03 min

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February 06, 2017

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, ,
1Division of Viral Diseases,Centers for Disease Control and Prevention

Chapters

  • 00:05Title
  • 01:04Swab Sampling, Virus Concentration, Viral RNA Extraction, and Purification
  • 02:36Midi Column Viral Nucleic Acid Extraction
  • 03:40Viral Nucleic Acid Concentration
  • 05:16Multiplex RT-qPCR Detection
  • 07:09Quantification of Norovirus in Swab Samples and Hemi-nested Conventional PCR Amplification
  • 08:11Results: Macrofoam-based Swab Sampling Method Effectively Detects Norovirus Outbreaks
  • 09:24Conclusion

Summary

Automatic Translation

Automatic Translation

A macrofoam based sampling methodology was developed and evaluated for the detection and quantification of norovirus on environmental hard surfaces.

Tags

Swab SamplingNorovirusEnvironmental SurfaceMacrofoamSample CollectionSample StorageSample TransportLaboratory ProtocolSurface ContaminationRemediation StrategiesStainless SteelToilet SeatDoorknobUNEX BufferPBSTColiphage MS2Lysis SolutionEthanolMidi ColumnNucleic Acid Extraction

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