Journal
/
Immunology and Infection
/
Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

DOI:
10.3791/55235-v

•

09:07 min

•

January 20, 2017

•

, , , , , ,
1Department of Molecular and Biomedical Sciences,University of Maine, 2Graduate School of Biomedical Sciences and Engineering,University of Maine, 3School of Biology and Ecology,University of Maine, 4Division of Intramural Research, Immunity, Inflammation and Disease Laboratory,National Institute of Environmental Health Sciences, NIH, 5Department of Chemical and Biological Engineering,University of Maine

Chapters

  • 00:05Title
  • 00:44Systemic Influenza A Virus Infection at 48 Hr Post Fertilization
  • 03:07Localized, Swim Bladder IAV Infection of Tg(mpx:mCherry) Transgenic Zebrafish
  • 04:19Anti-viral Drug Treatment
  • 05:23Mounting Zebrafish for Imaging
  • 06:39Results: Anti-viral Drug Treatment of IAV Infection in Zebrafish
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

Systemic and localized zebrafish infection models for human influenza A virus are demonstrated. Using a systemic infection model, zebrafish can be used to screen antiviral drugs. Using a localized infection model, zebrafish can be used to characterize host immune cell responses.

Tags

Keywords: ZebrafishInfluenzaVirusAntiviralImmune ResponsePhagocytesInflammationHigh-throughputAPR-8X31NS1-GFPMicroinjectionDuct Of CuvierPosterior Cardinal VeinCirculatory SystemSystemic Infection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55235?article_type=v

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice
check_url/55235?article_type=v

A Genetic Screen to Isolate Toxoplasma gondii Host-cell Egress Mutants
check_url/55235?article_type=v

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
check_url/55235?article_type=v

Biomimetic Materials to Characterize Bacteria-host Interactions
check_url/55235?article_type=v

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface
check_url/55235?article_type=v

Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
check_url/55235?article_type=v

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
check_url/55235?article_type=v

Using a Bacterial Pathogen to Probe for Cellular and Organismic-level Host Responses
check_url/55235?article_type=v

Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
check_url/55235?article_type=v

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Read Article