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Biochemistry

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Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
 

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

Article DOI: 10.3791/55236
January 26th, 2017

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Summary January 26th, 2017

We describe here a novel, robust, and efficient tandem affinity purification (TAP) method for the expression, isolation, and characterization of protein complexes from eukaryotic cells. This protocol could be utilized for the biochemical characterization of discrete complexes as well as the identification of novel interactors and post-translational modifications that regulate their function.

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