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Genetics

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Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
 

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Article DOI: 10.3791/55255
May 24th, 2017

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Summary May 24th, 2017

Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.

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