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Mapeando as Interações RNA-ARN Globalmente Usando Psoraleno Biotinilado
JoVE Journal
Genetics
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JoVE Journal Genetics
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
DOI:

11:32 min

May 24, 2017

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Chapters

  • 00:05Title
  • 01:12Treatment of HeLa Cells with Biotinylated Psoralen RNA Extraction, and Fragmentation
  • 02:25RNA Size Selection and Elution
  • 04:41Enrichment of RNA Crosslinking Regions
  • 05:57Proximity Ligation
  • 07:34Reverse Crosslinking of Biotinylated Psoralen, and Amplification of cDNA
  • 09:13The SPLASH Workflow and Representative Results
  • 10:50Conclusion

Summary

Automatic Translation

Aqui, detalhamos o método de equação de S de sorgo de P soralen reticulado, L igado e Híbridos H elegidos (SPLASH), que permite o mapeamento genômico de interações intramoleculares e intermoleculares RNA-RNA in vivo . SPLASH pode ser aplicado para estudar os interactomas de RNA de organismos, incluindo leveduras, bactérias e seres humanos.

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