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Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
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Chapters
- 00:05Title
- 00:51Transfer of hiPSCs Grown on iMEF Feeder Cells onto Extracellular Matrix for Propagation
- 02:56Small-scale Passaging of hiPSCs on Matrix-coated Plates
- 04:05Preparation of Chamber Slides and Coverslips for Seeding of hiPSCs for Immunocytochemistry
- 06:04Immunostaining of hiPSCs with Pluripotency Markers
- 08:12Results: Representative Images of iPSC Colonies and Immunocytochemical Characterization of Human iPSCs
- 09:10Conclusion
Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.
