/
/
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:57Preparation of Paper Layers and Nylon Membrane Layer
- 02:51Creating Hydrophobic Barriers in the Printed Layer
- 03:53Preparation of Adhesive Layers and Bqacking of Device Layers with Adhesive
- 04:52Treatment of Conjugate Layer with Reagents for Immunoassays Prior to Device Assembly
- 05:38Treatment of Lateral Channel and Capture Layer with Reagents for Immunoassays Prior to Device Assembly
- 06:52Assembly of Three-dimensional Paper-based Microfluidic Devices
- 08:33Performing a Paper-based Immunoassay
- 09:17Results: Fabrication of Paper-based Immunoassays
- 10:33Conclusion
We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.
