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Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

DOI:
10.3791/55292-v

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11:23 min

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February 23, 2017

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, , , ,
1Research Center for Applied Sciences,Academia Sinica

Chapters

  • 00:05Title
  • 01:20Fabrication of the PC-embedded Top Layer
  • 02:59Assembly of the Device
  • 04:42Microfluidic Cell Migration Assay
  • 08:38Results: Characterization of Chemical and Oxygen Gradients Established within the Microfluidic Device, and Study of Cell Migration Under Gradient Combinations
  • 10:35Conclusion

Summary

Automatic Translation

Automatic Translation

The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.

Tags

Keywords: Polydimethylsiloxane-polycarbonateMicrofluidic DeviceCell MigrationChemical GradientOxygen GradientPDMSPolycarbonateMammalian CellsCell MotilityCell-based Drug TestingCancer Cell Metastasis

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