Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

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Biology

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Biology

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JoVE Journal Biology

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

DOI:

10.3791/55324-v

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07:40 min

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February 05, 2018

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Aurelia C. Kucera, Britt J. Heidinger

¹Environmental and Conservation Sciences Program,North Dakota State University, ²Department of Biological Sciences,North Dakota State University

Chapters

00:04Title
00:41Semen Collection from a Passerine Bird Using Cloacal Massage
03:34Extraction of DNA from Bird Semen
06:32Results: Success by Bird Species and DNA Electrophoresis
07:08Conclusion

Summary

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This protocol describes cloacal massage, a non-invasive manual technique for collecting semen from birds, and DNA extraction from avian sperm using a commercial extraction kit with modifications.

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm

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