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JoVE Journal
Developmental Biology

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Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
 

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

Article DOI: 10.3791/55335-v 11:55 min February 24th, 2017
February 24th, 2017

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Summary

Zebrafish retinal regeneration has mostly been studied using fixed retinas. However, dynamic processes such as interkinetic nuclear migration occur during the regenerative response and require live-cell imaging to investigate the underlying mechanisms. Here, we describe culture and imaging conditions to monitor Interkinetic Nuclear Migration (INM) in real-time using multiphoton microscopy.

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Keywords: Retinal Explant Live-cell Imaging Multiphoton Microscopy Zebrafish Interkinetic Nuclear Migration Retinal Regeneration Muller Glia Microglial Behavior Phagocytosis Agarose FluoroDish
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