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Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model

Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model

DOI:
10.3791/55339-v

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08:03 min

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June 11, 2017

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, ,
1Department of Neurobiology, Physiology, and Behavior,University of California, Davis, 2Department of Physiology and Membrane Biology,University of California, Davis, 3Faculty of Kinesiology and Physical Education,University of Toronto

Chapters

  • 00:05Title
  • 00:43Prepare Brushite Anchors
  • 02:08Form Engineered Ligaments
  • 04:33Obtain Human Serum
  • 05:12Tensile Testing Engineering Ligaments
  • 06:13Results: Exercise-conditioned Media Treatment of Engineered Ligament Conctructs
  • 07:12Conclusion

Summary

Automatic Translation

Automatic Translation

We present a model of ligament tissue in which three-dimensional constructs are treated with the human exercise-conditioned serum and analyzed for collagen content, function, and cellular biochemistry.

Tags

Keywords: LigamentExerciseNutritionSerumIn VitroIn VivoBrushite CementAnchorFibroblastsThrombinAprotininAminohexanoic Acid

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