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Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
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Immunology and Infection
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JoVE Journal Immunology and Infection
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

DOI:
10.3791/55345-v

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08:35 min

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January 07, 2019

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, ,
1Institute of Immunology, Section Molecular Immunology,University of Heidelberg

Chapters

  • 00:04Title
  • 00:38Pan-leukocyte Preparation and Immune Synapse Induction and Staining
  • 02:45Flow Cytometric Image Acquisition
  • 03:39Data Analysis
  • 05:54Results: Representative CD3 and F-actin Immune Synapse Enrichment Analysis
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a complete workflow for the qualitative and quantitative analysis of immune synapses between primary human T cells and antigen-presenting cells. The method is based on imaging flow cytometry, which allows the acquisition and evaluation of several thousand cell images within a relatively short period of time.

Tags

Keywords: Immune SynapseLeukocyte CommunicationImaging Flow CytometryT Cell AnalysisStaphylococcus Aureus Enterotoxin B (SEB)Raji CellsPan-leukocytesParaformaldehyde FixationSaponin PermeabilizationFluorophore-conjugated AntibodiesFlow Cytometry

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