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Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
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1Center for Translational Medicine Research and Development,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 2Department of Orthopedic Surgery,Maastricht UMC+, 3Department of Orthopaedic Surgery,University Hospital RWTH, 4Research Laboratory for Biomedical Optics and Molecular Imaging,Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences
Chapters
- 00:05Title
- 00:58Scaffold Preparation
- 02:30Lentivirus Particle Release and Transduction Capacity
- 03:51Crtitical Bone Defect Model and Scaffold Transplantation
- 05:21In Vivo Imaging of Angiogenesis within the Bone Defect
- 07:00Results: 3D Scaffolds Promote Angiogenesis in Bone Defects
- 08:33Conclusion
Here, we present a protocol to visualize blood vessel formation in vivo and in real-time in 3D scaffolds by multiphoton microscopy. Angiogenesis in genetically modified scaffolds was studied in a murine calvarial critical bone defect model. More new blood vessels were detected in the treatment group than in controls.
