In this work, we report a new method to study protein-protein interactions using a conductimetric biosensor based on the hybrid β-lactamase technology. This method relies on release of protons upon hydrolysis of β-lactams.
Vandevenne, M., Dondelinger, M., Yunus, S., Freischels, A., Freischels, R., Crasson, O., Rhazi, N., Bogaerts, P., Galleni, M., Filée, P. The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions. J. Vis. Exp. (132), e55414, doi:10.3791/55414 (2018).