The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Maryl&#232;ne Vandevenne; Mathieu Dondelinger; Sami Yunus; Astrid Freischels; R&#233;gine Freischels; Oscar Crasson; Noureddine Rhazi; Pierre Bogaerts; Moreno Galleni; Patrice Fil&#233;e

doi:10.3791/55414

Journal

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Bioengineering

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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

8,975 Views

•

08:06 min

•

February 01, 2018

DOI:

10.3791/55414-v

DOI:

10.3791/55414-v

•

08:06 min

February 01, 2018

•

8962 Views

Marylène Vandevenne*¹, Mathieu Dondelinger*¹, Sami Yunus², Astrid Freischels¹, Régine Freischels¹, Oscar Crasson¹, Noureddine Rhazi¹, Pierre Bogaerts³, Moreno Galleni¹, Patrice Filée⁴

¹Life Science Department,University of Liège ²Institute of Condensed Matter and Nanoscience,Catholic University of Louvain ³Laboratory of Clinical Microbiology,Catholic University of Louvain ⁴Biotechnology Department,CER Group

Summary

In this work, we report a new method to study protein-protein interactions using a conductimetric biosensor based on the hybrid β-lactamase technology. This method relies on release of protons upon hydrolysis of β-lactams.

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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

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