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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
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Bioengineering
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JoVE Journal Bioengineering
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

DOI:
10.3791/55414-v

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08:06 min

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February 01, 2018

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, , , , , , , , ,
1Life Science Department,University of Liège, 2Institute of Condensed Matter and Nanoscience,Catholic University of Louvain, 3Laboratory of Clinical Microbiology,Catholic University of Louvain, 4Biotechnology Department,CER Group

Chapters

  • 00:05Title
  • 00:52Sensor Preparation and Regeneration
  • 02:04Binding Assay Performed on the Sensor
  • 04:45Results: Measurement of Conducticity Cganges Upon Antibiotic Hydrolysis by the Immobilization Chimeric β-Lactamase
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

In this work, we report a new method to study protein-protein interactions using a conductimetric biosensor based on the hybrid β-lactamase technology. This method relies on release of protons upon hydrolysis of β-lactams.

Tags

Beta-lactamaseConductimetric BiosensorBiomolecular InteractionsAntibody-antigen InteractionsChimeric Beta-lactamaseProton ReleaseImmunosensorsHybrid ProteinProtein Sample PreparationElectrode PreparationElectrode RegenerationHen-egg-white LysozymePolyanilinePhosphate BufferBlocking SolutionBlaP-cAb-Lys3BlaP

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