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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
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Immunology and Infection
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JoVE Journal Immunology and Infection
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

DOI:
10.3791/55417-v

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08:09 min

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March 24, 2017

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,
1Institute of Cellular and Organismic Biology,Academia Sinica

Chapters

  • 00:05Title
  • 00:55Preparation of Monoclonal Antibody
  • 02:24Construction and Expression of Serially Truncated Recombinant Proteins
  • 03:45Dot-blot Hybridization
  • 06:24Results: Recognition of the Epitope Region by Dot-blot Hybridization
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.

Tags

Keywords: Monoclonal AntibodyLinear B-cell EpitopePeptide ScanningDot-blot HybridizationAntigen-antibody InteractionRecombinant ProteinE. Coli ExpressionProtein PurificationProtein G Affinity Chromatography

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