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Journal / Immunology and Infection / Live-Imaging af antifungal aktivitet af humane…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Live-Imaging af antifungal aktivitet af humane primære neutrofiler og monocytter i Reaktion på
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Live-Imaging af antifungal aktivitet af humane primære neutrofiler og monocytter i Reaktion på

Article DOI: 10.3791/55444-v • 12:29 min • April 19th, 2017
April 19th, 2017 •
1, 1, 1, 2, 2, 2, 1
1Aberdeen Fungal Group, MRC Centre for Medical Mycology, Institute of Medical Sciences, University of Aberdeen, 2Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, US

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Her beskriver vi en protokol til at vurdere antifungal aktivitet af primære humane immunceller i realtid under anvendelse af fluorescerende Aspergillus reporter konidier sammenholdt med live-cell video mikroskopi og flowcytometri. Genererede data give indsigt i værtens celle- Aspergillus interaktioner såsom fungicid aktivitet, fagocytose, cellevandring og hæmning af svampevækst.

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Immunologi , Levende celler antifungal aktivitet FLARE humane leukocytter vært-patogen interaktion.
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