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Genetics

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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
 

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Article doi: 10.3791/55446
March 12th, 2017

Summary March 12th, 2017

We describe a basic experimental approach for analysis of termination of transcription by RNA polymerase II in vivo using BrUTP by the strand-specific transcription run-on (TRO) approach in budding yeast. This protocol can be extended to study transcription termination by other RNA polymerases both in yeast and higher eukaryotes.

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