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High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

DOI:
10.3791/55448-v

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13:28 min

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May 16, 2017

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, , , , , , , ,
1Department of Structural Biochemistry,Max Planck Institute of Molecular Physiology, 2Department of Biochemistry and Molecular Biology,The University of Texas Medical School at Houston

Chapters

  • 00:05Title
  • 01:16Micrograph Alignment and Particle Picking
  • 03:482D Classification and Initial 3D Modeling
  • 05:513D Model-refinement
  • 07:533D Sorting and Local Resolution Estimation
  • 10:46Results: High-resolution 3D Modeling of TcdA1
  • 12:19Conclusion

Summary

Automatic Translation

Automatic Translation

This paper presents a protocol for processing cryo-EM images using the software suite SPHIRE. The present protocol can be applied for nearly all single particle EM projects that target near-atomic resolution.

Tags

Keywords: Single Particle AnalysisElectron Cryo-microscopySPHIREMacromolecular ComplexesHigh-resolution Cryo-EMParticle Box SizeCTF EstimationMotion CorrectionDose-weightedDose-unweightedParticle Picking

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