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JoVE Journal
Genetics

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Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
 

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Article doi: 10.3791/55451
January 22nd, 2018

Summary January 22nd, 2018

Here, we present a protocol for tracing genomic DNA (gDNA) contamination in RNA samples. The presented method utilizes primers specific for the internal transcribed spacer region (ITS) of ribosomal DNA (rDNA) genes. The method is suited for reliable and sensitive detection of DNA contamination in most eukaryotes and prokaryotes.

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