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Genetics

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JoVE Journal Genetics
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
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Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Article DOI: 10.3791/55451-v • 13:16 min • January 22nd, 2018
January 22nd, 2018 •
1, 1, 2, 3, 4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

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Summary
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Here, we present a protocol for tracing genomic DNA (gDNA) contamination in RNA samples. The presented method utilizes primers specific for the internal transcribed spacer region (ITS) of ribosomal DNA (rDNA) genes. The method is suited for reliable and sensitive detection of DNA contamination in most eukaryotes and prokaryotes.

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DNA Contamination RNA Samples Ribosomal DNA Quantitative Real-time PCR Internal Transcribed Spacer Region Poaceae Family Reversed Transcription Quantitative Real-time PCR Multicopy Genes Cost-effective ITS1 And ITS2 17-18S 5.8S And 25-28S Subunits Aeluropus Littoralis RDNA-based Primer Design Species-specific Primers Universal Primers Guanidinium Thiocyanate Agarose Gel
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