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Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening
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Bioengineering
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JoVE Journal Bioengineering
Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

DOI:
10.3791/55461-v

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10:39 min

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April 15, 2017

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, , , , , ,
1Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center,University Medical Center Hamburg-Eppendorf and DZHK (German Center for Cardiovascular Research)

Chapters

  • 00:05Title
  • 01:05Engineering Heart Tissue (EHT) Generation
  • 03:40Contraction Analysis
  • 05:48Drug Screening
  • 08:20Results: Representative EHT Contraction Analysis
  • 09:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we show the generation of human engineered heart tissue from induced pluripotent stem cells (hiPSC)-derived cardiomyocytes. We present a method to analyze contraction force and exemplary alteration of contraction pattern by the hERG channel inhibitor E-4031. This method shows high level of robustness and suitability for cardiac drug screening.

Tags

Cardiac Drug Safety ScreeningContractile ForceIn Vitro Contraction AnalysisDisease ModelingSafety PharmacologyGene VariantsAgarosePDMS RacksCardiomyocytesFibrinogenThrombinEHT Medium

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