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Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
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Genetics
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JoVE Journal Genetics
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

DOI:
10.3791/55463-v

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06:56 min

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March 31, 2017

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, , , , ,
1Department of Plant and Microbial Biology,University of California Berkeley, 2John Innes Centre,Norwich Research Park, 3Department of Plant and Microbial Biology,University of Zürich, 4USDA-ARS Sunflower and Plant Biology Research Unit

Chapters

  • 00:05Title
  • 00:32Genomic DNA Purification
  • 01:22DNA Extraction I and Precipitation I
  • 03:30DNA Extraction II and Precipitation II
  • 05:44Results: Gels Distinguish Acceptable, Marginal and Failed Genomic DNA Samples
  • 06:33Conclusion

Summary

Automatic Translation

Automatic Translation

Described here is a method for the extraction, purification, and quality control of genomic DNA from the obligate biotrophic fungal pathogen, powdery mildew, for use in long-read genome sequencing.

Tags

Keywords: DNA ExtractionGenomic DNAPowdery MildewLong-read SequencingConidiaLysis BufferSarkosylChloroform Isoamyl AlcoholIsopropanolEthanolTE BufferRNase

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