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JoVE Journal Genetics
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
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Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Article DOI: 10.3791/55463-v • 06:56 min • March 31st, 2017
March 31st, 2017 •
*1,2, *1, 3, 4, 3, 1
1Department of Plant and Microbial Biology, University of California Berkeley, 2John Innes Centre, Norwich Research Park, 3Department of Plant and Microbial Biology, University of Zürich, 4USDA-ARS Sunflower and Plant Biology Research Unit
* These authors contributed equally

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Summary
Automatic Translation

Described here is a method for the extraction, purification, and quality control of genomic DNA from the obligate biotrophic fungal pathogen, powdery mildew, for use in long-read genome sequencing.

Tags

High Molecular Weight Genomic DNA Powdery Mildew Long-read Sequencing DNA Isolation Plant Pathogenic Fungal Biology Extraction Technique Lysis Buffer Sarkosyl Chloroform Isoamyl Alcohol Centrifuge Aqueous Layer
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