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Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
 

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Article doi: 10.3791/55466
March 16th, 2017

Summary March 16th, 2017

Labeling the extracellular domain of a membrane protein with a pH sensitive fluorophore, superecliptic pHluorin (SEP), allows subcellular localization, expression, and trafficking to be determined. Imaging SEP-labeled proteins with total internal reflection fluorescence microscopy (TIRFM) enables the quantification of protein levels in the peripheral ER and plasma membrane.

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