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Biology

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Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
 

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Article DOI: 10.3791/55466 09:59 min March 16th, 2017
March 16th, 2017

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Summary

Labeling the extracellular domain of a membrane protein with a pH sensitive fluorophore, superecliptic pHluorin (SEP), allows subcellular localization, expression, and trafficking to be determined. Imaging SEP-labeled proteins with total internal reflection fluorescence microscopy (TIRFM) enables the quantification of protein levels in the peripheral ER and plasma membrane.

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Fluorescence Imaging PHluorin-tagged Receptors Subcellular Localization Protein Trafficking Intracellular Distribution Membrane Protein Vesicle Delivery Protein Expression Real-time Imaging High Resolution Microscopy PH-sensitive Fluorophor Extracellular Solution TIRF Microscopy Epifluorescence Mode Exposure Time EM Gain Laser Beam
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