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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
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JoVE Journal Genetics
Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

DOI:
10.3791/55473-v

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10:19 min

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April 08, 2017

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1Department of Pediatrics,University of Pittsburgh School of Medicine, Children’s Hospital of Pittsburgh

Chapters

  • 00:05Title
  • 00:56Getting Started with Galaxy
  • 03:28Data Analysis with Tuxedo Suite
  • 06:19Gene Ontology (GO) Term Analyses Using DAVID
  • 07:56Results: Transcriptome Dictated by the Transcription Elongation Factor TCER-1, that Extends Lifespan of Germline-less C. elegans
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Galaxy and DAVID have emerged as popular tools that allow investigators without bioinformatics training to analyze and interpret RNA-Seq data. We describe a protocol for C. elegans researchers to perform RNA-Seq experiments, access and process the dataset using Galaxy and obtain meaningful biological information from the gene lists using DAVID.

Tags

Keywords: C. ElegansRNA SequencingTranscriptomic AnalysisTuxedo SuiteGalaxy ProjectBioinformaticsDifferential Gene ExpressionGene OntologyNGS RNA AnalysisFASTQData UploadWorkflow

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