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Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus
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Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus

DOI:
10.3791/55483-v

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07:23 min

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June 15, 2017

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, , , , , , ,
1Department of Frontier Health Sciences, Graduate School of Human Health Sciences,Tokyo Metropolitan University, 2Department of Anatomy,Tokyo Medical University, 3Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering,Saitama University

Chapters

  • 00:05Title
  • 00:54Whole-mount Immunohistochemistry
  • 05:12Results: Representative Neurofilament+ Nerve Fiber Extrahepatic Bilary Tract Staining in S. murinus
  • 06:33Conclusion

Summary

Automatic Translation

Automatic Translation

A whole-mount immunohistochemical approach, to visualize neurofilament protein expression in the extrahepatic biliary tract in Suncus murinus. is presented here. This protocol can be used to analyze the innervation of all visceral organs in S. murinus or other species.

Tags

Keywords: Whole-mount ImmunohistochemistryBiliary TractSuncus MurinusNerve DistributionVisceral OrgansNeoprene LatexGallbladderOrthoperiodic AcidPapillonParaformaldehydeSucrose Incubations

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