A whole-mount immunohistochemical approach, to visualize neurofilament protein expression in the extrahepatic biliary tract in Suncus murinus. is presented here. This protocol can be used to analyze the innervation of all visceral organs in S. murinus or other species.
Ren, K., Dai, Y., Yi, K., Kinoshita, M., Itoh, M., Sakata, I., Sakai, T., Yi, S. Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus. J. Vis. Exp. (124), e55483, doi:10.3791/55483 (2017).