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JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Ensaio da Actividade da Proteína Quinase com ATP Radiomarcado
A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction
Journal (Biochemistry)
A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
Journal (Biochemistry)
Isolation of Active Caenorhabditis elegans Nuclear Extract and Reconstitution for In Vitro Transcription
 
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Ensaio da Actividade da Proteína Quinase com ATP Radiomarcado

Article DOI: 10.3791/55504
May 26th, 2017 •
1, 1, 1
1Department of Pharmacology, University of Texas Southwestern Medical Center

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May 26th, 2017

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As cinases de proteínas são altamente evoluídas sinalizando enzimas e andaimes que são críticos para a transdução de sinal inter e intracelular. Nós apresentamos um protocolo para medir a atividade de quinase através do uso de adenosina trifosfato radiomarcado ([γ- 32 P] ATP), um método confiável para auxiliar na elucidação da regulação da sinalização celular.

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