Journal
/
Genetics
/
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

DOI:
10.3791/55505-v

•

08:56 min

•

June 27, 2017

•

, , , , , , ,
1Division of Nephrology, First Department of Integrated Medicine,Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University

Chapters

  • 00:05Title
  • 00:56Peritoneum Sample Collection
  • 01:54Purifying Total RNAs from Peritoneal Membrane Samples
  • 05:36Reverse Transcription of Total RNA
  • 06:44qRT-PCR of miRNA
  • 08:06Results: Upregulated miRNAs in the Peritoneal Membrane of Peritoneal Fibrosis Rats
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for the detection of microRNA expression in rat peritoneal membrane using quantitative real-time reverse-transcription polymerase chain reaction. This method is suitable for studying the microRNA expression profile in rat peritoneal membrane in several pathological conditions.

Tags

Keyword Extraction: MiRNAPeritoneal MembraneQuantitative Real-time PCRRNA PurificationRatHomogenizationPhenol-guanidineChloroformEthanolSpin Column

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55505?article_type=v

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
check_url/55505?article_type=v

Detection of Copy Number Alterations Using Single Cell Sequencing
check_url/55505?article_type=v

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents
check_url/55505?article_type=v

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
check_url/55505?article_type=v

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
check_url/55505?article_type=v

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
check_url/55505?article_type=v

CRISPR-Mediated Reorganization of Chromatin Loop Structure
check_url/55505?article_type=v

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
check_url/55505?article_type=v

Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR
check_url/55505?article_type=v

High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology

Read Article