Journal
/
Cancer Research
/
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues

In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues

DOI:
10.3791/55529-v

•

10:36 min

•

March 21, 2017

•

, , , , ,
1MTA-SE Pathobiochemistry Research Group,Semmelweis University, 2Department of Medical Chemistry, Molecular Biology and Pathobiochemistry,Semmelweis University, 3MTA-ELTE Research Group of Peptide Chemistry,Eötvös Loránd University

Chapters

  • 00:05Title
  • 01:07GnRH Uptake
  • 02:39Type I GnRH Receptor Expression
  • 05:39Fluorescence Microscopy Imaging
  • 06:26FACS-assisted GnRH Quantification
  • 08:00Results: GnRH I Uptake and Type I GnRH Receptor Expression in Pharynx Carcinoma Cells
  • 09:25Conclusion

Summary

Automatic Translation

Automatic Translation

Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines.

Tags

Keywords: In Vitro ImagingQuantificationDrug Targeting EfficiencyFluorescently Labeled GnRH AnaloguesHuman Cancer Cell CulturesTargeted Anti-cancer TherapyGnRH ReceptorsConfocal MicroscopyFlow CytometryGnRH FITCPreheated Treating MediaFixing SolutionFluorescent ProbeMounting Medium

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55529?article_type=v

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
check_url/55529?article_type=v

Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System
check_url/55529?article_type=v

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting
check_url/55529?article_type=v

A Data Integration Workflow to Identify Drug Combinations Targeting Synthetic Lethal Interactions
check_url/55529?article_type=v

In Vivo Targeting of Xenografted Human Cancer Cells with Functionalized Fluorescent Silica Nanoparticles in Zebrafish
check_url/55529?article_type=v

In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
check_url/55529?article_type=v

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo
check_url/55529?article_type=v

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
check_url/55529?article_type=v

Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer Cells
check_url/55529?article_type=v

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Read Article