The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

William J. Comstock; Edwin Huh; Reiley Weekes; Connor Watson; Tianyang Xu; Pieter C. Dorrestein; Robert A. Quinn

doi:10.3791/55532

/

The WinCF Model – An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

The WinCF Model – An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections

9,181 Views

•

06:57 min

•

May 08, 2017

DOI:

10.3791/55532-v

DOI:

10.3791/55532-v

•

06:57 min

May 08, 2017

•

9159 Views

William J. Comstock¹, Edwin Huh², Reiley Weekes², Connor Watson², Tianyang Xu², Pieter C. Dorrestein¹, Robert A. Quinn¹

¹Skaggs School of Pharmacy and Pharmaceutical Sciences,University of California, San Diego ²Department of Mechanical and Aerospace Engineering,University of California, San Diego

Summary

The mucus plugged airways of cystic fibrosis (CF) patients are an ideal environment for microbial pathogens to thrive. The manuscript describes a novel method for studying the CF lung microbiome in an environment that mimics where they cause disease and how alterations of chemical conditions can drive microbial dynamics.