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Immunology and Infection
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A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva

DOI:
10.3791/55591-v

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07:35 min

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July 05, 2017

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, , , ,
1Lee Kong Chian School of Medicine,Nanyang Technological University, 2Singapore Eye Research Institute, 3Singapore National Eye Center, 4Duke-NUS Medical School, 5Yong Loo Lin School of Medicine

Chapters

  • 00:05Title
  • 01:14Ocular Cell Impression Cytology Collection and Flow Cytometric Phenotyping
  • 05:48Results: Representative Healthy Human Ocular T Cells Subsets
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

The exposed normal ocular surface consists of cornea and conjunctiva. Epithelial cells, goblet cells and immune cells are present in the conjunctiva. Here, a non-invasive, technique of impression cytology is described using an impression cytology device and flow cytometry to analyze immune cells in the conjunctiva.

Tags

Keywords: Impression CytologyOcular SurfaceImmune CellsNon-invasiveCell PhenotypingOcular InflammationOcular ToxicologyOcular FibrosisFlow CytometryConjunctiva

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