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Samtidig isolering af cardiomyocytter af høj kvalitet, endotelceller og fibroblaster fra en voksen rottehjerte
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Samtidig isolering af cardiomyocytter af høj kvalitet, endotelceller og fibroblaster fra en voksen rottehjerte

Article DOI: 10.3791/55601-v • 09:38 min • May 19th, 2017
May 19th, 2017 •
1, 1, 1
1Internal Medicine, Cardiology and Angiology, University Hospital Giessen

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Flere protokoller er blevet udviklet og beskrevet for isolering af forskellige hjertecelletyper fra et rottehjerte. Her beskrives en optimeret protokol, der gør det muligt at isolere højkvalitets hovedkardiale celletyper (kardiomyocytter, endotelceller og fibroblaster) fra et enkelt præparat, hvilket reducerer forsøgsudgifterne.

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Cellulærbiologi udgave 123 endotelceller kardiomyocytter fibroblaster Langendorff isolering cellekultur
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