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JoVE Journal
Biochemistry

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High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
 

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Article doi: 10.3791/55623
May 13th, 2017

Summary May 13th, 2017

A protocol for high-precision FRET experiments at the single molecule level is presented here. Additionally, this methodology can be used to identify three conformational states in the ligand-binding domain of the N-methyl-D-aspartate (NMDA) receptor. Determining precise distances is the first step towards building structural models based on FRET experiments.

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