Biochemistry
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High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Chapters
Summary May 13th, 2017
A protocol for high-precision FRET experiments at the single molecule level is presented here. Additionally, this methodology can be used to identify three conformational states in the ligand-binding domain of the N-methyl-D-aspartate (NMDA) receptor. Determining precise distances is the first step towards building structural models based on FRET experiments.
Transcript
The overall goal of this protocol is to perform high precision inter-dye distance measurements using multi-parameter fluorescence detection of F
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