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Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples
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Cancer Research
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JoVE Journal Cancer Research
Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

DOI:
10.3791/55634-v

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09:57 min

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May 10, 2017

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, , , ,
1Institute of Immunobiology,Kantonsspital St. Gallen, 2Internal Medicine V,Medical University of Innsbruck, 3Tyrolean Cancer Research Institute (TKFI), 4Medical Clinic III, Oncology, Hematology, Immunoncology and Rheumatology,University Clinic Bonn (UKB)

Chapters

  • 00:05Title
  • 01:41Cell Preparation
  • 02:51Sample Preparation and Staining
  • 05:44Flow Cytometry Analysis
  • 07:24Results: Identification and Characterization of Side Population Cells
  • 08:20Conclusion

Summary

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Methods allowing the characterization and isolation of stem cell populations from biological samples are critical for the advance of stem cell-targeted treatments in cancer and beyond. Here, we provide a detailed protocol for cancer stem cell isolation using the dye-triggered side population phenotype.

Tags

Keywords: DNA DyeSide PopulationCancer Stem CellsABC Drug TransportersCell CultureCell SuspensionTrypsin-EDTAHemocytometerViolet DyeInhibition Controls

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