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A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

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Biology

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JoVE Journal Biology

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

A Graphical User Interface for Software-assisted Tracking of Protein Concentration in Dynamic Cellular Protrusions

DOI:

10.3791/55653-v

•

08:12 min

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July 11, 2017

•

Tanumoy Saha, Isabel Rathmann, Milos Galic

¹DFG Cluster of Excellence ‘Cells in Motion’, (EXC 1003), Institute of Medical Physics and Biophysics,University of Muenster

Chapters

00:05Title
00:43Cell Culture
01:42Image Acquisition and Analysis
06:54Results: Filopodia Analysis in COS Cells Transfected with f-actin
07:37Conclusion

Summary

Automatic Translation

We present a software solution for semi-automated tracking of relative protein concentration along the length of dynamic cellular protrusions.

Tags

Keywords: Protein Concentration Dynamic Cellular Protrusions Graphical User Interface Software-assisted Tracking Filopodial Dynamics Spatially Resolved Protein Localization Adaptive Shape Tracking Algorithm Cell Biology Filopodia Extension And Retraction Transfected Cells Image Acquisition Signal-to-noise Ratio Microscopy Image Pre-processing Graphical User Interface

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