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JoVE Journal
Developmental Biology

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Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
 

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

Article doi: 10.3791/55700
August 3rd, 2017

Summary August 3rd, 2017

We present a protocol that combines cell isolation and whole-cell patch-clamp recording to measure the electrical properties of the primary dissociated epithelial cells from the rat cauda epididymides. This protocol allows for investigation of the functional properties of primary epididymal epithelial cells to further elucidate the physiological role of the epididymis.

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