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Developmental Biology

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JoVE Journal Developmental Biology
Gravação de grampos-grampo de células inteiras de células epiteliais primárias isoladas do epidídimo
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Gravação de grampos-grampo de células inteiras de células epiteliais primárias isoladas do epidídimo

Article DOI: 10.3791/55700
August 3rd, 2017 •
1,2,3, 1,2,3, 1,3, 4, 1
1School of Life Science and Technology, ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University

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August 3rd, 2017

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Apresentamos um protocolo que combina o isolamento celular e a gravação de grampos-grampos de células inteiras para medir as propriedades elétricas das células epiteliais dissociadas primárias dos epidídimos da cauda do rato. Este protocolo permite a investigação das propriedades funcionais das células epiteliais primárias epidídmicas para elucidar ainda mais o papel fisiológico do epidídimo.

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