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Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response
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Chapters
- 00:05Title
- 01:07Preparing Equipment for Microinjection
- 02:46Making a Tumor Cell Suspension
- 04:22Injecting Tumor Suspension into the Fourth Ventricle of a 2-dpf Embryo
- 07:25Results: Brain Tumor Transplantation in Zebrafish
- 08:57Conclusion
The transplantation of cancer cells is an important tool for the identification of cancer mechanisms and therapeutic responses. Current techniques depend on immune-incompetent animals. Here, we describe a method to transplant zebrafish tumor cells into immune-competent embryos for the long-term analysis of tumor cell behavior and in vivo drug responses.
