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Immunology and Infection
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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
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Immunology and Infection
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JoVE Journal Immunology and Infection
Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

DOI:
10.3791/55760-v

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07:58 min

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May 30, 2017

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, , , ,
1Department of Biological Sciences,Plymouth State University

Chapters

  • 00:05Title
  • 01:28Preparation and Use of Frozen Stock Cultures
  • 02:46Growth of Overnight Cultures in Liquid Medium
  • 03:23Phenotypic Assays for Biotype Classification via Spotting
  • 04:04Motility Assay
  • 04:48Voges-Proskauer (VP) Assay
  • 05:47Results: Reliable Distinction Between Biotypes
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes proper Vibrio cholerae maintenance techniques in addition to a series of biochemical assays, collectively utilized for quick and reliable differentiation between clinical and environmental V. cholerae biotypes in a laboratory setting.

Tags

Keywords: Vibrio CholeraeClinical IsolatesEnvironmental IsolatesBiotypingGenotypic CharacterizationPhenotypic CharacterizationBiochemical AssaysMetabolic CapabilitiesEnteric BacteriaLaboratory TechniquesCulture MaintenanceCryogenic StorageAgar PlatingLiquid CultureInoculation

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