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Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications

Article DOI: 10.3791/55778-v • 08:41 min • July 14th, 2017
July 14th, 2017 •
1, 1, 2, 1,2
1Pharmasan Labs, Inc., 2NeuroScience, Inc.

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Summary
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A selective and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method coupled with an efficient solid phase extraction on a mixed-mode cation-exchange (MCX) 96-well microplate was developed for the measurement of free 3-nitrotyrosine (3-NT) in human urine with high throughput, which is suitable for clinical applications.

Tags

Miniaturized Solid Phase Extraction LC-MS/MS Detection 3-nitrotyrosine Human Urine Clinical Applications Biomarker Oxidative Stress Sensitivity Selectivity Sample Preparation Specific Determination Low Levels Endogenous 3-nitrotyrosine Constitution Two-dimensional LC Oxidative Stress Investigation Pathological Conditions Inflammatory Disorders Neurodegenerative Disorders Antioxidant Treatment Monitoring Effectiveness Vortexing Urine Samples Standards QC Samples
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