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JoVE Journal
Bioengineering

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Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry
 

Isolation of Primary Murine Retinal Ganglion Cells (RGCs) by Flow Cytometry

Article doi: 10.3791/55785
July 5th, 2017

Summary July 5th, 2017

Millions of people suffer from retinal degenerative diseases that result in irreversible blindness. A common element of many of these diseases is the loss of retinal ganglion cells (RGCs). This detailed protocol describes the isolation of primary murine RGCs by positive and negative selection with flow cytometry.

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