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Developmental Biology

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JoVE Journal Developmental Biology
A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
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A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

Article DOI: 10.3791/55795-v • 12:41 min • August 3rd, 2017
August 3rd, 2017 •
1, 1, 1, 1
1State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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Summary
Automatic Translation

This work demonstrates a novel approach to assess the proliferation, differentiation, and vessel-forming potential of vascular endothelial stem cells (VESCs) through mammary fat pad transplantation followed by whole-mount tissue preparation for microscopic observation. A lineage tracing strategy to investigate the behavior of VESCs in vivo is also presented.

Tags

Mammary Fat Pad Transplantation Vessel Generation Vascular Endothelial Stem Cells Whole-mount Tissue Preparation Microscopic Observation Stemness Properties Angiogenic Environment Transplanted Endothelium Cells Angiogenic Factors Vessel Remodeling Tumor Environment Cell Potency Tissue Digestion Actin-GFP Reporter Mice Scalpel Incision Inguinal Mammary Glands
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