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Journal / Developmental Biology / Lysmedieret reversibel modulering af den…
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JoVE Journal Developmental Biology
Lysmedieret reversibel modulering af den mitogenaktiverede proteinkinasevej under celledifferentiering og
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Lysmedieret reversibel modulering af den mitogenaktiverede proteinkinasevej under celledifferentiering og

Article DOI: 10.3791/55823-v • 09:32 min • June 15th, 2017
June 15th, 2017 •
*1, *2, 1, 1, 1, 2, 2, 1,3,4
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, 3Neuroscience Program, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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Denne protokol beskriver en optogenetisk strategi til modulering af mitogenaktiveret proteinkinase (MAPK) aktivitet under celledifferentiering og Xenopus embryonal udvikling. Denne metode muliggør reversibel aktivering af MAPK-signalvejen i pattedyrcellekultur og i multicellulære levende organismer, som Xenopus- embryoner, med høj rumlig og tidsmæssig opløsning.

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Developmental Biology Optogenetics protein-protein interactions PC12 cell differentiation, Embryonisk udvikling kryptokrom CRY2-CIBN bicistronisk Raf / MEK / ERK kinase signaleringskaskade
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