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Biochemistry

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In Vitro Polymerization of F-actin on Early Endosomes
 

In Vitro Polymerization of F-actin on Early Endosomes

Article doi: 10.3791/55829
August 28th, 2017

Summary August 28th, 2017

Early endosome functions depend on F-actin polymerization. Here, we describe a microscopy-based in vitro assay that reconstitutes the nucleation and polymerization of F-actin on early endosomal membranes in test tubes, thus rendering this complex series of reactions amenable to biochemical and genetic manipulations.

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