Journal
/
Biochemistry
/
Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain

Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain

DOI:
10.3791/55835-v

•

09:35 min

•

October 24, 2017

•

, ,
1Department of Biochemistry,Emory School of Medicine

Chapters

  • 00:05Title
  • 00:42Homogenization and Fractionation
  • 04:54Western Blotting
  • 07:48Results: Detection of AD-related Detergent-insoluble Protein Aggregates from the Human Postmortem Brain
  • 09:05Conclusion

Summary

Automatic Translation

Automatic Translation

An abbreviated fractionation protocol for the enrichment of detergent-insoluble protein aggregates from human postmortem brain is described.

Tags

Keywords: Detergent-insoluble Protein AggregatesHuman Postmortem BrainNeurodegenerationMisfolded Protein AggregatesGray MatterLow-salt BufferSodium ChlorideSarkosylSonicationProtein Concentration

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/55835?article_type=v

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
check_url/55835?article_type=v

Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
check_url/55835?article_type=v

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum
check_url/55835?article_type=v

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
check_url/55835?article_type=v

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
check_url/55835?article_type=v

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
check_url/55835?article_type=v

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
check_url/55835?article_type=v

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
check_url/55835?article_type=v

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
check_url/55835?article_type=v

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Read Article