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In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption
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In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

DOI:
10.3791/55846-v

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10:36 min

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June 20, 2017

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, , ,
1Department of Anatomy and Cell Biology,University of Illinois at Chicago

Chapters

  • 00:05Title
  • 01:14Culturing hCMEC/D3 Immortalized Endothelial Cells
  • 02:08Preparation of Mouse Brain Endothelial Cells (BECs)
  • 04:35Single BEC Culture Assay
  • 06:24Triple Culture Assay
  • 08:13Quantification
  • 08:44Results: Effects of oAβ and EGF in Single- and Triple-culture Meshwork Disruption Assays
  • 09:51Conclusion

Summary

Automatic Translation

Automatic Translation

Maintaining blood-brain barrier coverage is key for the homeostasis of the central nervous system. This protocol describes in vitro techniques to delineate the fundamental and pathological processes that modulate blood-brain barrier coverage.

Tags

Keywords: Blood-brain BarrierIn Vitro AssayMesh-like Vessel FormationVessel DisruptionAlzheimer’s DiseaseNeurodegenerative DisordersHCMEC/D3 CellsMouse Brain Endothelial CellsPapainDNaseCell CultureCell PassagingCentrifugation

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