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Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation
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Chapters
- 00:05Title
- 01:28Tissue Fixation
- 03:54Post-fixation of NCFPE Specimens and Fluorescence In Situ Hybridization (FISH)
- 07:37Results: Data Analysis of FISH and Real-time Reverse Transcription PCR for Corresponding FFPE and NCFPE Tissue Sections
- 08:53Conclusion
Fluorescence in-situ hybridization (FISH) is often required in combination with histopathology and molecular diagnostics for selection of therapy in personalized medicine. A novel non-cross-linking, formalin-free tissue fixative that allows high quality morphologic, molecular and FISH analyses from the same specimen by addition of a post-fixation step before FISH is presented.
