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Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
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Cancer Research
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JoVE Journal Cancer Research
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model

Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model

DOI:
10.3791/55890-v

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09:03 min

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October 16, 2017

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, ,
1Department of Pathology and Laboratory Medicine,Weill Cornell Medicine

Chapters

  • 00:05Title
  • 00:46Collection and Concentration of Viruses Expressing Metastatic Factor
  • 02:26Viral Titer Determination
  • 04:37Intracardiac Injection
  • 07:24Results: Detection of Metastatic Pancreatic β-cells by Immunostaining
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to demonstrate a novel somatic gene transfer system utilizing RIP-Tag; RIP-tva mouse model to study the function of genes in metastasis. The avian retroviruses are delivered intracardiacally to ensure gene transfer into pre-malignant, noninvasive lesions of pancreatic β cells in adult mice.

Tags

Keywords: Metastatic FactorsGene TransferRIP-Tag; RIP-tva Mouse ModelPancreatic Beta Cell Precursor LesionsVirus Producing DF1 CellsViral SupernatantUltracentrifugationViral TiterSerial DilutionDF1 Cells

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